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Introduction to molecular ecology
Course description
This is a practically oriented course providing the basics for DNA analyses in any kind of free living organism. Steps included are DNA extraction, PCR, DNA sequencing, primer design, microsatellite analyses, phylogenetic reconstructions and population genetic analyses of DNA sequences and polyallelic markers. Computer programs that will be used inlcude BioEdit, MEGA, DNAsp, FSTAT and STRUCTURE.
Course organization
The course starts with an excursion for sample collections followed by hands-on experience with DNA extraction and sequencing of organelle DNA (mitochondrial or chloroplast DNA) and down stream analyses of sequence alignment and phylogenetic analyses.
The second part of the course is about genetic variation within species and will be illustrated by amplification of microsatellite DNA, genotype interpretations and population genetic analyses. In parallel with the practical parts, there will be lectures giving the theoretical background to the exercises.
Pre-requisites
The course is open to all GENECO students regardless of affiliation or research agenda.
Organizers:
Helena Westerdahl helena.westerdahl@zooekol.lu.se
Lars Råberg lars.raberg@zooekol.lu.se
Time-period
January 18-February 4, 2011
Literature
Course Book: "Introduction to Molecular Ecology" 2nd ed. 2008. Trevor Beebee and Graham Rowe. Oxford University Press
Seminar papers & recommended reading
Last modified 8 Jul 2010
Helena Westerdahl
Assistant Professor
MEMEG
Phone:
+46 46-2223669
E-mail:
Helena.Westerdahl@biol.lu.se
Phone:
+46 46-2223766
E-mail:
Lars.Raberg@biol.lu.se