A fast and scalable kymograph alignment algorithm for nanochannel-based optical DNA mappings.
Författare
Summary, in English
Optical mapping by direct visualization of individual DNA molecules, stretched in nanochannels with sequence-specific fluorescent labeling, represents a promising tool for disease diagnostics and genomics. An important challenge for this technique is thermal motion of the DNA as it undergoes imaging; this blurs fluorescent patterns along the DNA and results in information loss. Correcting for this effect (a process referred to as kymograph alignment) is a common preprocessing step in nanochannel-based optical mapping workflows, and we present here a highly efficient algorithm to accomplish this via pattern recognition. We compare our method with the one previous approach, and we find that our method is orders of magnitude faster while producing data of similar quality. We demonstrate proof of principle of our approach on experimental data consisting of melt mapped bacteriophage DNA.
Avdelning/ar
- Beräkningsbiologi och biologisk fysik - Genomgår omorganisation
- Fasta tillståndets fysik
- Atomfysik
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- NanoLund: Centre for Nanoscience
Publiceringsår
2015
Språk
Engelska
Publikation/Tidskrift/Serie
PLoS ONE
Volym
10
Issue
4
Dokumenttyp
Artikel i tidskrift
Förlag
Public Library of Science (PLoS)
Ämne
- Condensed Matter Physics
- Biophysics
- Other Physics Topics
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1932-6203