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Capture of bacteriocins directly from non-clarified fermentation broth using macroporous monolithic cryogels with phenyl ligands

In English

Författare

Summary, in English

The bacteriocin, sakacin P was produced by the bacteriocin-producing strain Lactobacillus sakei CCUG 42687 at 20 degrees C without pH control. The bacteriocin was captured directly from the fermentation broth using macroporous octyl- and phenyl-monolith columns. The large size of the interconnected macropores (up to 100 mu m) in the macroporous monolith allowed for direct fermentation broth processing with no clarification needed. Screening for optimal bacteriocin binding demonstrated that at pH 6.2 about 80% of the bacteriocin activity could be recovered with a purification factor of 150-160 in the cell-free eluate. Capture of bacteriocins from the fermentation broth using macroporous monoliths in the 96-well plate format presents a promising approach for rapid analytical isolation of bacteriocins from numerous samples.

Avdelning/ar

Publiceringsår

2007

Språk

Engelska

Sidor

786-793

Publikation/Tidskrift/Serie

Enzyme and Microbial Technology

Volym

40

Issue

4

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Industrial Biotechnology

Nyckelord

  • cryogel in microtiter plate
  • macroporous monolith
  • bacteriocin
  • integrated product adsorption

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0141-0229