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Analysis of protein expression in pure cell nuclei populations isolated from human breast cancer tissue by DNA flow cytometric sorting.

In English

Författare

Summary, in English

In this study, cell nuclei from aneuploid breast cancer samples were sorted with respect to DNA content into pure diploid and aneuploid fractions using flow cytometry. The nuclear proteins were then separated by one-dimensional gel electrophoresis (1D-PAGE) and differences in protein expression patterns, between diploid and aneuploid nuclei from the same tumours, were compared. Using a combination of peptide finger printing and peptide identification by MALDI-TOF mass spectrometry, we identified proteins and confirmed that the proteins were of nuclear origins. The results in this study add further information to the knowledge about the breast cancer disease complexity and heterogeneity at molecular level. For some of the tumours studied different nuclei protein patterns were obtained, in the diploid respective aneuploid nuclei populations, whilst other tumours did not show these differences.

Publiceringsår

2010

Språk

Engelska

Sidor

1111-1116

Publikation/Tidskrift/Serie

Journal of Proteomics

Volym

73

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Surgery
  • Cancer and Oncology

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1874-3919