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Gene delivery to adult neural stem cells

In English

Författare

Summary, in English

Neural stem cells may present an ideal route for gene therapy as well as offer new possibilities for the replacement of neurons lost to injury or disease. However, it has proved difficult to express ectopic genes in stem cells. We report methods to introduce genes into adult neural stem cells using viral and nonviral vectors in vitro and in vivo. Adenoviral and VSV-G-pseudotyped retroviral vectors are more efficient than plasmid transfection or VSV-G lentiviral transduction in vitro. We further show that adult neural stem cells can be directed to a neuronal fate by ectopic expression of neurogenin 2 in vitro. Plasmids can be delivered in vivo when complexed with linear polyethyleneimine, and gene expression can be targeted specifically to neural stem or progenitor cells by the use of specific promoters. These techniques may be utilized both to study the function of various genes in the differentiation of neural stem cells to specific cell fates and, ultimately, for gene therapy or to generate specific differentiated progeny for cell transplantation. (C) 2002 Elsevier Science (USA).

Avdelning/ar

Publiceringsår

2002

Språk

Engelska

Sidor

34-39

Publikation/Tidskrift/Serie

Experimental Cell Research

Volym

279

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Academic Press

Ämne

  • Neurosciences

Status

Published

Forskningsgrupp

  • Neurobiology

ISBN/ISSN/Övrigt

  • ISSN: 1090-2422