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Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography

Författare:
  • Anna Mölder
  • Mikael Sebesta
  • Mats Gustafsson
  • Lennart Gisselson
  • Anette Gjörloff Wingren
  • Kersti Alm
Publiceringsår: 2008
Språk: Engelska
Sidor: 240-247
Publikation/Tidskrift/Serie: Journal of Microscopy
Volym: 232
Nummer: 2
Dokumenttyp: Artikel i tidskrift
Förlag: Wiley-Blackwell

Sammanfattning

Manual cell counting is time consuming and requires a high

degree of skill on behalf of the person performing the count.

Here we use a technique that utilizes digital holography,

allowing label-free and completely non-invasive cell counting

directly in cell culture vessels with adherent viable cells.

The images produced can provide both quantitative and

qualitative phase information from a single hologram.

The recently constructed microscope HolomonitorTM (Phase

Holographic Imaging AB, Lund, Sweden) combines the

commonly used phase contrast microscope with digital

holography, the latter giving us the possibility of achieving

quantitative information on cellular shape, area, confluence

and optical thickness. This project aimed at determining the

accuracy and repeatability of cell counting measurements

using digital holography compared to the conventional

manual cell counting method using a haemocytometer. The

collected datawere also used to determine cell size and cellular

optical thickness.Theresults showthat digital holography can

be used for non-invasive automatic cell counting as precisely

as conventional manual cell counting

Nyckelord

  • Biological Sciences
  • Electrical Engineering, Electronic Engineering, Information Engineering
  • Cell analysis
  • cellular optical thickness
  • cell size
  • cell counting
  • digital holography

Övriga

Published
  • Electromagnetic theory-lup-obsolete
  • ISSN: 0022-2720

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