Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays

Summary, in English

Antibody microarrays enable extensive protein expression profiling, and provide a valuable complement to DNA microarray-based gene expression profiling. In this study, we used DotScan™ antibody microarrays that contain antibodies against 82 different cell surface antigens, to determine phenotypic protein expression profiles for human B cell sub-populations. We then demonstrated that the B cell protein profile can be used to delineate the relationship between normal B cells and malignant counterparts. Principle component analysis showed that the lymphomas did not cluster with the normal memory B cells or germinal centre B cells, but they did cluster with germinal centre founder cells and naïve B cells.

• Författare: Peter Ellmark; Carl-Magnus Högerkorp; Sara Ek; Larissa Beelov; Mattias Berglund; Richard Rosenquist; Richard Christopherson; Carl Borrebaeck
• Avdelning/ar: Institutionen för immunteknologi
• Publiceringsår: 2008
• Språk: Engelska
• Sidor: 98-106
• Publikation/Tidskrift/Serie: Cancer Letters
• Volym: 265
• Länkar: http://dx.doi.org/10.1016/j.canlet.2008.02.006
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Cancer and Oncology
• Status: Published
• Forskningsgrupp: Carl Borrebaeck
• ISBN/ISSN/Övrigt: ISSN: 1872-7980