Du är här

DRhoGEF2 and Diaphanous Regulate Contractile Force during Segmental Groove Morphogenesis in the Drosophila Embryo.

Författare:
Publiceringsår: 2008
Språk: Engelska
Sidor: 1883-1892
Publikation/Tidskrift/Serie: Molecular biology of the cell
Volym: 19
Dokumenttyp: Artikel

Sammanfattning

Monitoring Editor: Marianne Bronner-Fraser Morphogenesis of the Drosophila embryo is associated with dynamic rearrangement of the Actin cytoskeleton mediated by small GTPases of the Rho family. These GTPases act as molecular switches that are activated by guanine nucleotide exchange factors. One of these factors, DRhoGEF2, plays an important role in the constriction of Actin filaments during pole cell formation, blastoderm cellularization and invagination of the germlayers. Here we show that DRhoGEF2 is equally important during morphogenesis of segmental grooves, which become apparent as tissue infoldings during midembryogenesis. Examination of DRhoGEF2-mutant embryos indicates a role for DRhoGEF2 in the control of cell shape changes during segmental groove morphogenesis. Overexpression of DRhoGEF2 in the ectoderm recruits Myosin II to the cell cortex and induces cell contraction. At groove regression DRhoGEF2 is enriched in cells posterior to the groove that undergo apical constriction indicating that groove regression is an active process. We further show that the Formin Diaphanous is required for groove formation and strengthens cell junctions in the epidermis. Morphological analysis suggests that Dia regulates cell shape in a way distinct from DRhoGEF2. We propose that DRhoGEF2 acts through Rho1 to regulate acto-myosin constriction but not Diaphanous-mediated F-Actin nucleation during segmental groove morphogenesis.

Disputation

Nyckelord

  • Medicine and Health Sciences

Övrigt

Published
Yes
  • ISSN: 1059-1524

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen

LERU logo U21 logo