Exposure of HEp-2 Cells to Stress Conditions Influences Antinuclear Antibody Reactivity.

Summary, in English

This study of stress-related antinuclear antibody (ANA) reactivity was undertaken with the objective of improving clinical ANA testing. ANA was determined by parallel enzyme-linked immunosorbent assays of crude nuclear protein antigen extracted from HEp-2 cells either grown under optimal conditions (providing nonstress ANA antigen) or exposed to stress (providing stress ANA antigen). The stress stimuli used were gamma radiation (causing DNA damage) and a hypertonic environment (causing apoptosis). Signs of stress-related ANA reactivity were seen among connective tissue disease (CTD) patients (including patients with systemic lupus erythematosus; mixed CTD; calcinosis, Reynaud's phenomenon, esophageal motility disorders, sclerodactyly, and telangiectasia; scleroderma; and Sjögren's syndrome): 11% showed stress-positive ANA (i.e., a significantly stronger ANA reactivity with the extract from stressed cells), whereas 21% showed a markedly weaker reaction with the stress antigen. In contrast, among ANA screening patient sera, with no diagnosis of CTD, the fraction showing stress-positive ANA was higher (7 to 8%, depending on the type of stress) than among those showing a lower reactivity with stress antigen (1.5 to 2.5%). Only one serum among 89 (1%) tested sera from healthy individuals showed a stress-related ANA reaction. This demonstration of stress-related ANA suggests a means to improve the performance of clinical ANA testing.

• Författare: Liping Du; Sachiko Fukushima; Annahita Sallmyr; Rolf Manthorpe; Anders Bredberg
• Avdelning/ar: Klinisk mikrobiologi, Malmö; Reumatologi och molekylär skelettbiologi
• Publiceringsår: 2002
• Språk: Engelska
• Sidor: 287-294
• Publikation/Tidskrift/Serie: Clinical and Diagnostic Laboratory Immunology
• Volym: 9
• Issue: 2
• Fulltext: - 615 kB; Download statistics
• Länkar: http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11874865&dopt=Abstract; http://dx.doi.org/10.1128/CDLI.9.2.287-294.2002
• Dokumenttyp: Artikel i tidskrift
• Förlag: American Society for Microbiology
• Ämne: Microbiology in the medical area; Rheumatology and Autoimmunity
• Nyckelord: Epitopes; Epithelial Cells/cytology/*immunology; Enzyme-Linked Immunosorbent Assay; DNA Damage; Autoimmune Diseases/diagnosis/immunology; Apoptosis/*immunology; Antigen-Antibody Reactions; Antinuclear/*analysis/immunology; Cultured; Antibodies; Human; Hypertonic Solutions; Osmotic Pressure; Support; Non-U.S. Gov't; Tumor Cells
• Status: Published
• Forskningsgrupp: Clinical Microbiology, Malmö
• ISBN/ISSN/Övrigt: ISSN: 1071-412X