Regulation of phospholipase C-gamma 2 via phosphatidylinositol 3-kinase in macrophages.
Författare
Summary, in English
Phospholipase C-gamma (PLC-gamma) isoforms are thought to be activated by both tyrosine phosphorylation and phosphatidylinositol 3,4,5 trisphosphate (PtdIns 3,4,5 P(3)), the product of phosphatidylinositol 3-kinase (PtdIns 3-kinase). In this study, we show that stimulation of mouse macrophages with either zymosan beads or bacteria (Prevotella intermedia) induced tyrosine phosphorylation of PLC-gamma 2. Zymosan stimulation also induced translocation to membrane and cytoskeleton fractions, which was inhibited by the PtdIns 3-kinase inhibitors wortmannin and LY 294002. However, the tyrosine phosphorylation of PLC-gamma 2 induced by zymosan was not affected by the inhibitors wortmannin and LY 294002. In contrast to zymosan and bacteria, PLC-gamma 2 was not phosphorylated by stimulation with lipopolysaccharide (LPS), phorbol ester or calcium ionophore. Moreover, the PLC-gamma 1 isoform was not detected in mouse macrophages. These data indicate that PtdIns 3-kinase is critical for the translocation but not for the tyrosine phosphorylation of PLC-gamma 2 in mouse macrophages and that the latter may be insufficient for enzyme activation.
Publiceringsår
2002
Språk
Engelska
Sidor
169-173
Publikation/Tidskrift/Serie
Cellular Signalling
Volym
14
Issue
2
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Microbiology
Nyckelord
- Chromones : pharmacology
- Cytoskeleton : enzymology
- Dose-Response Relationship Drug
- Female
- Macrophages : drug effects : enzymology
- Isoenzymes : metabolism
- Mice
- Morpholines : pharmacology
- Phosphotyrosine : metabolism
- Phosphorylation
- Phospholipase C : metabolism
- Prevotella intermedia
- Protein Transport
- Signal Transduction
- Support Non-U.S. Gov't
- Zymosan : pharmacology
- Cells Cultured
- Cell Membrane : enzymology
- Animal
- Androstadienes : pharmacology
- 1-Phosphatidylinositol 3-Kinase : antagonists & inhibitors : physiology
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1873-3913