Inhibitory effect on arterial injury-induced neointimal formation by adoptive B-cell transfer in Rag-1 knockout mice.

Summary, in English

We investigated the effect of B-cell reconstitution in immune-deficient Rag-1 knockout (KO) mice subjected to arterial injury. After 21 days, injury induced a 4- to 5-fold increase in neointimal formation in Rag-1 KO mice fed normal chow compared with wild-type (WT) mice (0.020+/-0.0160 [n=8] versus 0.0049+/-0.0022 [n=8] mm(2), respectively; P<0.05) and in western-type diet-fed Rag-1 KO mice compared with WT mice (0.0312+/-0.0174 [n=7] versus 0.0050+/-0.0028 [n=6] mm(2), respectively; P<0.05). To investigate the role of B cells in response to injury, Rag-1 KO mice were reconstituted with B cells derived from the spleens of WT mice, with donors and recipients on the same diet. Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed normal chow) reduced neointimal formation compared with the effect in unreconstituted Rag-1 KO mice (0.0076+/-0.0039 [n=9] versus 0.020+/-0.0160 [n=8] mm(2), respectively; P<0.05). Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed a western diet) reduced neointimal formation compared the effect in Rag-1 KO mice (0.0087+/-0.0037 [n=8] versus 0.0312+/-0.0174 [n=7] mm(2), respectively; P<0.05). Injured carotid arteries from reconstituted Rag-1 KO mice had detectable IgM and IgG, indicating viable transfer of B cells. The results suggest that B cells modulate the response to arterial injury.

• Författare: Paul Dimayuga; Bojan Cercek; Sumito Oguchi; Gunilla Nordin Fredrikson; Juliana Yano; Prediman K Shah; Stefan Jovinge; Jan Nilsson
• Avdelning/ar: Kardiovaskulär forskning - immunitet och ateroskleros; Stamcellscentrum (SCC)
• Publiceringsår: 2002
• Språk: Engelska
• Sidor: 644-649
• Publikation/Tidskrift/Serie: Arteriosclerosis, Thrombosis and Vascular Biology
• Volym: 22
• Issue: 4
• Länkar: http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11950704&dopt=Abstract; http://dx.doi.org/10.1161/01.ATV.0000012455.62765.BF
• Dokumenttyp: Artikel i tidskrift
• Förlag: Lippincott Williams & Wilkins
• Ämne: Cardiac and Cardiovascular Systems
• Nyckelord: Knockout; Mice; Male; Immunoglobulin M : blood; Immunoglobulin G : blood; Cellular; Immunity; Homeodomain Proteins : drug effects; Diet; Cholesterol : blood; Carotid Arteries : pathology; Carotid Arteries : immunology; B-Lymphocytes : transplantation; B-Lymphocytes : immunology; Adoptive Transfer; Animal; Models; Support; Non-U.S. Gov't; Tunica Intima : growth & development; Tunica Intima : immunology; Tunica Intima : injuries
• Status: Published
• Forskningsgrupp: Cardiovascular Research - Immunity and Atherosclerosis
• ISBN/ISSN/Övrigt: ISSN: 1524-4636