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The human V-preB promoter is a target for coordinated activation by early B cell factor and E47.

Författare:
Publiceringsår: 2002
Språk: Engelska
Sidor: 5130-5138
Publikation/Tidskrift/Serie: Journal of Immunology
Volym: 168
Nummer: 10
Dokumenttyp: Artikel
Förlag: American Association of Immunologists

Sammanfattning

The development of mature B lymphoid cells involves a highly orchestrated regulation of stage- and lineage-specific genes. In this study, we report an analysis of the human surrogate L chain VpreB promoter. The promoter has an overall homology of 56% to the mouse counterpart and displays a preB cell-restricted activity in transient transfections in cell lines. The promoter harbors three independent binding sites for early B cell factor (EBF) as defined by EMSA and supershift experiments. These sites were important for the full function of the promoter in a preB cell line, and chromatin immunoprecipitation experiments indicate that EBF interacts with the promoter in vivo. In addition to this, ectopic expression of EBF induces the activity of a reporter gene under control of the VpreB promoter in epithelioid HeLa cells, an effect augmented by coexpression of the basic-helix-loop helix transcription factor E47. The ability to interact directly with E47 was shared by the promoters controlling the human mb-1 and B29 genes. These data indicate that the human VpreB promoter is a direct target for activation by EBF and E47 and that functional collaboration between these proteins may be of great importance in human B cell development.

Disputation

Nyckelord

  • Medicine and Health Sciences
  • Biology and Life Sciences
  • Trans-Activators : physiology
  • Non-U.S. Gov't
  • Support
  • Promoter Regions (Genetics) : immunology
  • Mice
  • Molecular Sequence Data
  • Membrane Glycoproteins : metabolism
  • Membrane Glycoproteins : genetics
  • Jurkat Cells
  • Immunoglobulin Variable Region : genetics
  • Human
  • Hematopoietic Stem Cells : immunology
  • Hematopoietic Stem Cells : metabolism
  • Hematopoietic Stem Cells : cytology
  • Hela Cells
  • B-Lymphocyte
  • Light Chain
  • Gene Rearrangement
  • Transformed
  • DNA-Binding Proteins : physiology
  • Animal
  • B-Lymphocytes : cytology
  • Transcription Factors : physiology
  • Cell Line
  • Cell Differentiation : immunology
  • Cell Differentiation : genetics
  • Base Sequence
  • B-Lymphocytes : metabolism
  • B-Lymphocytes : immunology

Övriga

Published
Yes
  • ISSN: 0022-1767

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