Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Separation and surveys of proteins of Helicobacter pylori.

In English

Författare

  • Ingrid Nilsson
  • Meeme Utt

Summary, in English

The analysis of Helicobacter pylori proteins is a demanding task for the elucidation of virulence factors, antigens and vaccines, all important for diagnosis, therapy and protection. In the "pre-genomic era" the purification of proteins was mostly performed by using various techniques such as detergent treatment of the bacterial cells, ultra-centrifugation, various chromatographic methods, antibody detection, N-terminal sequence determination and finally cloning and identification of the corresponding gene. In this review, the most representative methods used for purification, separation and identification of H. pylori proteins will be presented as well as some important developments in the "post-genomic era" that have improved the performance of these characterisation techniques.

Publiceringsår

2002

Språk

Engelska

Sidor

251-260

Publikation/Tidskrift/Serie

Journal of Chromatography. B

Volym

771

Issue

1-2

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Microbiology in the medical area

Nyckelord

  • Helicobacter pylori : chemistry
  • Bacterial
  • Genome
  • Two-Dimensional
  • Gel
  • Electrophoresis
  • Bacterial Proteins : isolation & purification
  • Bacterial Proteins : genetics
  • Proteome
  • Helicobacter pylori : genetics
  • Helicobacter pylori : pathogenicity

Status

Published

Forskningsgrupp

  • Celiac Disease and Diabetes Unit

ISBN/ISSN/Övrigt

  • ISSN: 1873-376X