The novel IgD binding protein from Moraxella catarrhalis induces human B lymphocyte activation and Ig secretion in the presence of Th2 cytokines.
Författare
Summary, in English
Moraxella IgD binding protein (MID) is a novel bacterial outer membrane protein with IgD-binding properties. MID was purified from the respiratory pathogen Moraxella catarrhalis and is here shown to have B cell stimulatory properties. Purified MID in the range of 0.01-0.1 microg/ml was optimal to induce a proliferative response in human PBL. MID coupled to Sepharose and formalin-fixed M. catarrhalis preparations induced similar proliferative responses in PBL cultures. MID or MID-Sepharose stimulated purified human peripheral B cells as measured by proliferation. In contrast, MID or MID-Sepharose did not activate T cells. Preincubation of purified B cells with anti-IgD Abs inhibited MID-Sepharose-induced B cell proliferation. The addition of IL-4 specifically induced IL-6 production in MID-Sepharose-activated B cells. IgM secretion was detected in B cell cultures stimulated with MID or MID-Sepharose and IL-2 for 10 days. Secretion of IgG and IgA was efficiently induced in cultures from purified B cells stimulated with the combination of MID or MID-Sepharose and IL-4, IL-10, and soluble CD40 ligand, suggesting that Th2-derived cytokines were required for optimal plasma cell generation. Taken together, MID has properties that make it an important tool to study IgD-targeted activation of B cells.
Publiceringsår
2002
Språk
Engelska
Sidor
5582-5588
Publikation/Tidskrift/Serie
Journal of Immunology
Volym
168
Issue
11
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
American Association of Immunologists
Ämne
- Immunology in the medical area
Nyckelord
- Immunoglobulin D : physiology
- Immunoglobulin A : biosynthesis
- Human
- Drug
- Dose-Response Relationship
- Cytokines
- Carrier Proteins : pharmacology
- B-Lymphocytes : immunology
- B-Lymphocytes : drug effects
- Interleukin-6 : biosynthesis
- Lymphocyte Transformation : drug effects
- Support
- Non-U.S. Gov't
- Th2 Cells : immunology
- Immunoglobulin G : biosynthesis
- Immunoglobulin M : biosynthesis
- Interleukin-2 : pharmacology
Status
Published
Forskningsgrupp
- Clinical Microbiology, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1550-6606