False positive results in class-specific rheumatoid factor (RF) assays due to interaction between RF and Fc fragments of anti-immunoglobulin indicator reagents
Författare
Summary, in English
A recently developed, simple immunological method, diffusion-in-gel ELISA, has been adapted for class-specific determination of rheumatoid factor (RF). Evidence was obtained of an analytical error due to interaction between RF and antigenic determinants on the Fc part of the indicator immunoglobulin (Ig) molecules used. This was eliminated by replacing the usual indicator reagent, complete Ig molecules, by conjugated Fab or F(ab')2 fragments. The findings imply that unless the RF-Fc interaction mentioned is avoided in the assaying technique, false positive results may be obtained for, e.g. IgG RF and IgA RF in sera containing IgM RF.
Publiceringsår
1983
Språk
Engelska
Sidor
171-182
Publikation/Tidskrift/Serie
Journal of Immunological Methods
Volym
58
Issue
1-2
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Immunology in the medical area
Nyckelord
- rheumatoid factor
- immunoassay
- immunoglobulin class
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1872-7905