Molecular cloning and sequence analysis of cDNA coding for the precursor of the human cysteine proteinase inhibitor cystatin C

Summary, in English

Recombinant cystatin C producing clones were isolated from a human placenta λgt11 cDNA library. The cDNA insert of one of the clones, containing 777 base pairs, encodes the complete mature cystatin C (120 amino acids) and a hydrophobic leader sequence of 26 amino acids, indicating an extracellular function of the inhibitor. The deduced protein sequence confirms the protein sequence of cystatin C isolated from human urine, but differs in one position from the sequence of the cystatin C fragment deposited as amyloid in hereditary cerebral hemorrhage with amyloidosis.

• Författare: Magnus Abrahamson; Anders Grubb; Isleifur Olafsson; Åke Lundwall
• Avdelning/ar: Avdelningen för klinisk kemi och farmakologi; Klinisk kemi, Malmö
• Publiceringsår: 1987
• Språk: Engelska
• Sidor: 229-233
• Publikation/Tidskrift/Serie: FEBS Letters
• Volym: 216
• Issue: 2
• Länkar: http://dx.doi.org/10.1016/0014-5793(87)80695-6
• Dokumenttyp: Artikel i tidskrift
• Förlag: Wiley-Blackwell
• Ämne: Biological Sciences
• Nyckelord: Enzyme inhibitor; Cysteine proteinase; Cerebral hemorrhage; Cystatin C; cDNA cloning; Nucleotide sequence
• Status: Published
• Forskningsgrupp: Clinical Chemistry, Malmö
• ISBN/ISSN/Övrigt: ISSN: 1873-3468