G proteins coupled to phospholipase C: molecular targets of long-term ethanol exposure

Summary, in English

Long-term ethanol exposure is known to inhibit bradykinin-stimulated phosphoinositide hydrolysis in cultures of neuroblastoma x glioma 108-15 cells. In the present study, [3H]bradykinin binding, GTP-binding protein function, and phospholipase C activity were assayed in cells grown for 4 days in 100 mM ethanol with the aim of elucidating the molecular target of ethanol on signal transduction coupled to inositol trisphosphate and diacylglycerol formation. Ethanol exposure reduced guanosine 5'-O-(3-thiotriphosphate) [GTP(S)]- and, to a lesser extent, NaF/AlCl3-stimulated phosphoinositide hydrolysis, whereas it had no effect on the enzymatic activity of a phosphatidylinositol 4,5-bisphosphate-specific phospholipase C. [3H]Bradykinin binding in the absence of GTP(S) was not influenced by ethanol exposure. However, the reduction in [3H]bradykinin binding seen in control cells after addition of GTP analogue was inhibited in cells grown in ethanol-containing medium. The results indicate that long-term ethanol exposure exerts its effects on receptor-stimulated phosphoinositide hydrolysis primarily at the level of the GTP-binding protein.

• Författare: Per Simonsson; F David Rodriguez; Niklas Loman; Christer Alling
• Avdelning/ar: Klinisk kemi, Malmö; Bröstcancer-genetik; Avdelningen för klinisk kemi och farmakologi
• Publiceringsår: 1991
• Språk: Engelska
• Sidor: 2018-2026
• Publikation/Tidskrift/Serie: Journal of Neurochemistry
• Volym: 56
• Issue: 6
• Länkar: http://dx.doi.org/10.1111/j.1471-4159.1991.tb03461.x
• Dokumenttyp: Artikel i tidskrift
• Förlag: Wiley-Blackwell
• Ämne: Neurosciences
• Nyckelord: Bradykinin; Ethanol; Phosphatidylinositol 4; 5-bisphosphate; Phospholipase C; GTP-binding protein; NG 108–15 cell line
• Status: Published
• Forskningsgrupp: Clinical Chemistry, Malmö
• ISBN/ISSN/Övrigt: ISSN: 1471-4159