Hydrophobic interaction chromatography of fibroblast proteoglycans

Summary, in English

We have investigated the hydrophobic properties of human skin fibroblast proteoglycans and related material by affinity chromatography on Octyl-Sepharose CL-4B in 4 M guanidinium hydrochloride (GdnHCl). Proteoglycans and related material could be separated into non-, medium and highly hydrophobic forms by elution with gradients of Triton X-100 in 4 M Gdn HCl. The non-hydrophobic material included endogenously produced glycosaminoglycan chains and oligosaccharides as well as an HS-proteoglycan with a 35 kDa core. The 65-70 kDa core (glypican-related) proteoglycans appeared among the highly hydrophobic ones, but variable proportions were seen both in the medium and the non-hydrophobic material. Other membrane-bound proteoglycans, like fibroglycan (45 kDa core) and the HS-proteoglycans with 90 and 130 kDa cores, as well as the CS/DS-proteoglycan with a 90 kDa core, were all of high hydrophobicity. There were also indications of a highly hydrophobic CS/DS-proteoglycan with a 45 kDa core. The extracellular proteoglycans, PG-L, PG-S1 and PG-S2, and the HS-proteoglycans with 350 and 250 kDa cores were all of medium hydrophobicity. These proteoglycans emerged in distinct positions when the column was eluted with a gradient of 3-[(3-cholamidopropyl)dimethylammonio]propanesulphonate.

• Författare: Artur Schmidtchen; Lars-Åke Fransson
• Avdelning/ar: Dermatologi och venereologi, Lund; Glykobiologigruppen
• Publiceringsår: 1993
• Språk: Engelska
• Sidor: 48-55
• Publikation/Tidskrift/Serie: Biomedical Chromatography
• Volym: 7
• Issue: 1
• Länkar: http://dx.doi.org/10.1002/bmc.1130070113
• Dokumenttyp: Artikel i tidskrift
• Förlag: John Wiley & Sons Inc.
• Ämne: Medicinal Chemistry; Dermatology and Venereal Diseases
• Status: Published
• Forskningsgrupp: Glycobiology
• ISBN/ISSN/Övrigt: ISSN: 0269-3879