A functional prothrombin gene product is synthesized by human kidney cells
Författare
Summary, in English
gamma -carboxylated polypeptides were detected in the human kidney by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues. An similar to 70-kDa gamma -carboxylated protein, subsequently identified as prothrombin, was isolated from the intracellular compartment of cultured human embryonic kidney (HEK293) cells by immunoaffinity chromatography on M3B-coupled resin. Immunohistochemical analyses demonstrated that prothrombin and another vitamin K-dependent protein, the growth arrest-specific protein 6, were detectable in human kidney. As in the liver, the kidney synthesizes prothrombin as a zymogen that can be cleaved by ecarin to an amidolytically active serine protease that is inhibited by hirudin, This demonstrates for the first time the de novo synthesis of a full-length, gamma -carboxylated, and functional prothrombin gene product by human kidney cells.
Avdelning/ar
Publiceringsår
2001
Språk
Engelska
Sidor
1036-1041
Publikation/Tidskrift/Serie
Biochemical and Biophysical Research Communications
Volym
280
Issue
4
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Biological Sciences
Nyckelord
- kidney
- prothrombin
- γ-carboxyglutamate
- HEK293 cells
- growth arrest-specific protein 6
- nephrolithiasis
Status
Published
Forskningsgrupp
- Clinical Chemistry, Malmö
- Pathology, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1090-2104