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Multicenter comparison trial of DNA extraction methods and PCR assays for detection of Chlamydia pneumoniae in endarterectomy specimens

Författare

  • Petra Apfalter
  • Francesco Blasi
  • Jens Boman
  • Charlotte A. Gaydos
  • Michael Kundi
  • Matthias Maass
  • Athanasios Makristathis
  • Adam Meijer
  • Reinhard Nadrchal
  • Kenneth Persson
  • Manfred L. Rotter
  • C. Y. William Tong
  • Gerold Stanek
  • Alexander M. Hirschl

Summary, in English

The reported rate of detection of Chlamydia pneumoniae DNA within atherosclerotic lesions by PCR varies between 0 and 100%. In this study, identical sets of coded experimental atheroma samples (n = 15) and spiked controls (n = 5) were analyzed by 16 test methods in nine centers by means of PCR. The positive controls were correctly identified to levels of 1, 0.1, and 0.01 inclusion bodies of C. pneumoniae/ml of tissue homogenate by 16 (100%), 11 (69%), and 3 (19%) of the test methods, respectively. Three out of 16 negative controls (19%) were rated positive. Positivity rates for atheroma samples varied between 0 and 60% for the different test methods, with the maximum concordant result for positivity being only 25% for one carotid artery sample. There was no consistent pattern of positive results among the various laboratories, and there was no correlation between the detection rates and the sensitivity of the assay used.

Publiceringsår

2001

Språk

Engelska

Sidor

519-524

Publikation/Tidskrift/Serie

Journal of Clinical Microbiology

Volym

39

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

American Society for Microbiology

Ämne

  • Microbiology in the medical area

Status

Published

Forskningsgrupp

  • Clinical Microbiology, Malmö

ISBN/ISSN/Övrigt

  • ISSN: 1098-660X