Interaction of metals and thiols in cell damage and glutathione distribution: potentiation of mercury toxicity by dithiothreitol
Författare
Summary, in English
In the present study, we have investigated the effects of extracellular redox status and metal/thiol interactions on glutathione distribution in HeLa cell cultures. No effects were seen on glutathione distribution after the addition of different thiols, whereas the pro-oxidant copper ions affected glutathione distribution in several ways. The addition of dithiothreitol (DTT) but not the other thiols potentiated the effects of mercury ions on glutathione distribution and cell toxicity. In the presence of DTT, increased intra- and extracellular glutathione concentrations were noted already at 0.05 micromol/l, which was below the previously reported toxicity threshold for mercury ions in blood. Likewise DTT potentiated the effects of copper ions on glutathione distribution and cell toxicity, whereas the addition of DTT to cell cultures with a non-metal thiol reactive agent (hydroquinone) or an oxidative agent (hydrogen peroxide) did not affect glutathione distribution or cell toxicity. Thus, it seems as the synergistic effects between DTT and thiol reactive agents only apply to metal ions.
Avdelning/ar
Publiceringsår
2001
Språk
Engelska
Sidor
93-100
Publikation/Tidskrift/Serie
Toxicology
Volym
156
Issue
2-3
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Medicinal Chemistry
- Pharmacology and Toxicology
Nyckelord
- Cell culture
- Copper ions
- Dithiothreitol
- Glutathione
- Homocysteine
- Hydrogen peroxide
- Hydroquinone
- Mercury ions
- N-acetylcysteine
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0300-483X