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Estimating the variation in S phase duration from flow cytometric histograms.

Publiceringsår: 2008
Språk: Engelska
Sidor: 40-49
Publikation/Tidskrift/Serie: Mathematical biosciences
Volym: 213
Nummer: 1
Dokumenttyp: Artikel
Förlag: Elsevier

Sammanfattning

A stochastic model for interpreting BrdUrd DNA FCM-derived data is proposed. The model is based on branching processes and describes the progression of the DNA distribution of BrdUrd-labelled cells through the cell cycle. With the main focus on estimating the S phase duration and its variation, the DNA replication rate is modelled by a piecewise linear function, while assuming a gamma distribution for the S phase duration. Estimation of model parameters was carried out using maximum likelihood for data from two different cell lines. The results provided quite a good fit to the data, suggesting that stochastic models may be a valuable tool for analysing this kind of data.

Disputation

Nyckelord

  • Mathematics and Statistics
  • Medicine and Health Sciences
  • Branching process
  • Cell cycle progression
  • S phase duration
  • Flow cytometry

Övriga

Published
Yes
  • ISSN: 0025-5564

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