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Allelic variants of streptokinase from Streptococcus pyogenes display functional differences in plasminogen activation.

In English

Författare

  • Jason D McArthur
  • Fiona C McKay
  • Vidiya Ramachandran
  • Priya Shyam
  • Amanda J Cork
  • Martina L Sanderson-Smith
  • Jason N Cole
  • Ulrika Ringdahl
  • Ulf Sjöbring
  • Marie Ranson
  • Mark J Walker
Visa allt

Summary, in English

A common mammalian defense mechanism employed to prevent systemic dissemination of invasive bacteria involves occlusion of local microvasculature and encapsulation of bacteria within fibrin networks. Acquisition of plasmin activity at the bacterial cell surface circumvents this defense mechanism, allowing invasive disease initiation. To facilitate this process, S. pyogenes secretes streptokinase, a plasminogen-activating protein. Streptokinase polymorphism exhibited by S. pyogenes isolates is well characterized. However, the functional differences displayed by these variants and the biological significance of this variation has not been elucidated. Phylogenetic analysis of ska sequences from 28 S. pyogenes isolates revealed 2 main sequence clusters (clusters 1 and 2). All strains secreted streptokinase, as determined by Western blotting, and were capable of acquiring cell surface plasmin activity after incubation in human plasma. Whereas culture supernatants from strains containing cluster 1 ska alleles also displayed soluble plasminogen activation activity, supernatants from strains containing cluster 2 ska alleles did not. Furthermore, plasminogen activation activity in culture supernatants from strains containing cluster 2 ska alleles could only be detected when plasminogen was prebound with fibrinogen. This study indicates that variant streptokinase proteins secreted by S. pyogenes isolates display differing plasminogen activation characteristics and may therefore play distinct roles in disease pathogenesis.-McArthur, J. D., McKay, F. C., Ramachandran, V., Shyam, P., Cork, A. J., Sanderson-Smith, M. L., Cole, J. N., Ringdahl, U., Sjöbring, U., Ranson, M., Walker, M. J. Allelic variants of streptokinase from Streptococcus pyogenes display functional differences in plasminogen activation.

Publiceringsår

2008

Språk

Engelska

Sidor

3146-3153

Publikation/Tidskrift/Serie

The FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Volym

22

Issue

9

Dokumenttyp

Artikel i tidskrift

Förlag

The Federation of American Societies for Experimental Biology

Ämne

  • Cell and Molecular Biology

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1530-6860