Du är här

Protein crystals can be incommensurately modulated

Författare:
  • Jeffrey J Lovelace
  • Cameron R Murphy
  • Lee Daniels
  • Kartik Narayan
  • Clarence E Schutt
  • Uno Lindberg
  • Christer Svensson (Dr)
  • Gloria E. O. Borgstahl
Publiceringsår: 2008
Språk: Engelska
Sidor: 600-605
Publikation/Tidskrift/Serie: Journal of Applied Crystallography
Volym: 41
Nummer: Part 3
Dokumenttyp: Artikel
Förlag: Blackwell Publishing

Sammanfattning

For a normal periodic crystal, the X-ray diffraction pattern can be described by an orientation matrix and a set of three integers that indicate the reciprocal lattice points. Those integers determine the spacing along the reciprocal lattice directions. In aperiodic crystals, the diffraction pattern is modulated and the standard periodic main reflections are surrounded by satellite reflections. The successful indexing and refinement of the main unit cell and q vector using TWINSOLVE, developed by Svensson (2003). Lund University, Sweden, are reported here for an incommensurately modulated, aperiodic crystal of a profilin: actin complex. The indexing showed that the modulation is along the b direction in the crystal, which corresponds to an 'actin ribbon' formed by the crystal lattice. Interestingly, the transition to the aperiodic state was shown to be reversible and the diffraction pattern returned to the periodic state during data collection. It is likely that the protein underwent a conformational change that affected the neighbouring profilin: actin molecules in such a way as to produce the observed modulation in the diffraction pattern. Future work will aim to trap the incommensurately modulated crystal state, for example using cryocooling or chemical crosslinking, thus allowing complete X-ray data to be collected.

Disputation

Nyckelord

  • Chemistry
  • Medicine and Health Sciences
  • modulated protein crystals
  • profilin
  • actin
  • modulated diffraction
  • satellite reflections
  • incommensurate crystallography

Övriga

Published
Yes
  • ISSN: 0021-8898

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

 

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen