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Extracytoplasmatic processes impaired by inactivation of the trxA (thioredoxin gene) in Bacillus subtilis

Publiceringsår: 2008
Språk: Engelska
Sidor: 4660-4665
Publikation/Tidskrift/Serie: Journal of Bacteriology
Volym: 190
Nummer: 13
Dokumenttyp: Artikel
Förlag: ASM American Society for Microbiology

Sammanfattning

The trxA gene is regarded as essential in Bacillus subtilis, but the roles of the TrxA protein in this gram-positive bacterium are largely unknown. Inactivation of trxA results in deoxyribonucleoside and cysteine or methionine auxotrophy. This phenotype is expected if the TrxA protein is important for the activity of the class Ib ribonucleotide reductase and adenosine-5'-phosphosulfate/3'-phosphoadenosine-5'-phosphosulfate reductase. We demonstrate here that a TrxA deficiency in addition causes defects in endospore and cytochrome c synthesis. These effects were suppressed by BdbD deficiency, indicating that TrxA in the cytoplasm is the primary electron donor to several different thiol-disulfide oxidoreductases active on the outer side of the B. subtilis cytoplasmic membrane.

Disputation

Nyckelord

  • Biology and Life Sciences

Övriga

Published
Yes
  • ISSN: 0021-9193 (print)
  • ISSN: 1098-5530 (online)

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