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Heterogeneity of homologously expressed Hypocrea jecorina (Trichoderma reesei) Cel7B catalytic module.

Författare:
Publiceringsår: 2004
Språk: Engelska
Sidor: 1266-1276
Publikation/Tidskrift/Serie: European Journal of Biochemistry
Volym: 271
Nummer: 7
Dokumenttyp: Artikel
Förlag: Blackwell Publishing

Sammanfattning

The catalytic module of Hypocrea jecorina (previously Trichoderma reesei) Cel7B was homologously expressed by transformation of strain QM9414. Post-translational modifications in purified Cel7B preparations were analysed by enzymatic digestions, high performance chromatography, mass spectrometry and site-directed mutagenesis. Of the five potential sites found in the wild-type enzyme, only Asn56 and Asn182 were found to be N-glycosylated. GlcNAc2Man5 was identified as the predominant N-glycan, although lesser amounts of GlcNAc2Man7 and glycans carrying a mannophosphodiester bond were also detected. Repartition of neutral and charged glycan structures over the two glycosylation sites mainly accounts for the observed microheterogeneity of the protein. However, partial deamidation of Asn259 and a partially occupied O-glycosylation site give rise to further complexity in enzyme preparations.

Disputation

Nyckelord

  • Chemistry
  • N-glycan
  • protein glycosylation
  • O-glycan
  • Tricho-derma reesei
  • cellulase.

Övrigt

Published
Yes
  • ISSN: 0014-2956

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