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Site-selective regulation of platelet-derived growth factor beta receptor tyrosine phosphorylation by T-cell protein tyrosine phosphatase.

Författare

  • Camilla Persson
  • Catrine Sävenhed
  • Annie Bourdeau
  • Michel L Tremblay
  • Boyka Markova
  • Frank D Böhmer
  • Fawaz G Haj
  • Benjamin G Neel
  • Ari Elson
  • Carl-Henrik Heldin
  • Lars Rönnstrand
  • Arne Ostman
  • Carina Hellberg

Summary, in English

The platelet-derived growth factor (PDGF) ß receptor mediates mitogenic and chemotactic signals. Like other tyrosine kinase receptors, the PDGF ß receptor is negatively regulated by protein tyrosine phosphatases (PTPs). To explore whether T-cell PTP (TC-PTP) negatively regulates the PDGF ß receptor, we compared PDGF ß receptor tyrosine phosphorylation in wild-type and TC-PTP knockout (ko) mouse embryos. PDGF ß receptors were hyperphosphorylated in TC-PTP ko embryos. Fivefold-higher ligand-induced receptor phosphorylation was observed in TC-PTP ko mouse embryo fibroblasts (MEFs) as well. Reexpression of TC-PTP partly abolished this difference. As determined with site-specific phosphotyrosine antibodies, the extent of hyperphosphorylation varied among different autophosphorylation sites. The phospholipase C1 binding site Y1021, previously implicated in chemotaxis, displayed the largest increase in phosphorylation. The increase in Y1021 phosphorylation was accompanied by increased phospholipase C1 activity and migratory hyperresponsiveness to PDGF. PDGF ß receptor tyrosine phosphorylation in PTP-1B ko MEFs but not in PTP ko MEFs was also higher than that in control cells. This increase occurred with a site distribution different from that seen after TC-PTP depletion. PDGF-induced migration was not increased in PTP-1B ko cells. In summary, our findings identify TC-PTP as a previously unrecognized negative regulator of PDGF ß receptor signaling and support the general notion that PTPs display site selectivity in their action on tyrosine kinase receptors.

Copyright © 2004, American Society for Microbiology. All Rights Reserved

Publiceringsår

2004

Språk

Engelska

Sidor

201-2190

Publikation/Tidskrift/Serie

Molecular and Cellular Biology

Volym

24

Issue

5

Dokumenttyp

Artikel i tidskrift

Förlag

American Society for Microbiology

Ämne

  • Medicinal Chemistry

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0270-7306