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Positively Regulated Glycerol/G3P-Dependent Bacillus subtilis Gene Expression System Based on Anti-Termination.

Författare:
Publiceringsår: 2009
Språk: Engelska
Sidor: 61-70
Publikation/Tidskrift/Serie: Journal of molecular microbiology and biotechnology
Volym: 17
Dokumenttyp: Artikel
Förlag: Karger Publishers

Sammanfattning

Plasmid pLALA was constructed for glycerol or glycerol-3-phosphate inducible plasmid-borne gene expression in Bacillus subtilis and closely related Gram-positive bacteria. Gene expression using pLALA is based on anti-termination of transcription and involves the B. subtilis GlpP protein that in the presence of glycerol-3-phosphate acts as an anti-terminator protein by binding to the 5'-untranslated region of glpD mRNA. Properties and the usefulness of the system, denoted LALA, were validated by inducible production in B. subtilis strainsof two water-soluble proteins (beta-galactosidase and a protein phospho-tyrosine phosphatase), and one integral membrane protein (heme A synthase). Advantages with LALA is that it is based on positive control, does not involve a DNA-binding protein, and that glycerol, a cheap and stable compound, can be used as inducer of gene expression.

Disputation

Nyckelord

  • Biology and Life Sciences
  • Anti-termination
  • glp
  • Glycerol inducible
  • Bacillus subtilis

Övriga

Published
Yes
  • ISSN: 1660-2412

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