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Enzyme conformational alterations detected by partition column chromatography

Författare:
Publiceringsår: 1996
Språk: Engelska
Sidor: 113-122
Publikation/Tidskrift/Serie: Journal of Chromatography B: Biomedical Sciences and Applications
Volym: 680
Nummer: 1-2
Dokumenttyp: Artikel
Förlag: Elsevier

Sammanfattning

In this paper, we demonstrate the ability of liquid-liquid partition chromatography (LLPC) to detect conformational alterations occurring in well-characterized enzymes. The conformational changes induced in dehydrogenases such as alcohol dehydrogenase (ADH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenases (LDH) and malate dehydrogenase (MDH) upon binding of ligand(s) were detectable by LLPC. The ligand-dependent equilibrium between two forms of citrate synthase (CS), glutamate-oxaloacetate transaminase (GOT), hexokinase (HK) and 3-phosphoglycerate kinase (PGK) could also be demonstrated. Furthermore, different conformational forms of some of the apoenzymes could also be detected and separated by LLPC. The results obtained here are discussed in relation to those obtained by other methods.

Disputation

Nyckelord

  • Chemistry
  • Partition column chromatography
  • Conformational changes
  • Enzymes

Övriga

Published
Yes
  • ISSN: 1570-0232

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