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Cell-surface charge and cell-surface hydrophobicity of collagen-binding Aeromonas and Vibrio strains

In English

Författare

  • F Ascencio
  • G Johansson
  • Torkel Wadström

Summary, in English

Abstract Partitioning in aqueous polymer two-phase systems of polyethylene glycol and dextran was used to detect and compare cell-surface charge and cell-surface hydrophobicity of Aeromonas hydrophila, A. caviae, A. sobria, Vibrio cholerae, and V. anguillarum strains. These strains have cell-surface components that bound either native or thermally denatured type I collagen (i.e., a mixture of the alpha1+alpha2 chains) and gelatin immobilized on latex beads. Our goals were: (1) to compare the possible relationship between the cell-surface charge/hydrophobicity and binding to collagen and (2) to evaluate the influence of the culture media on the expression of surface properties. There was no apparent relationship between cell-surface charge, cell-surface hydrophobicity, and binding to collagen. The expression of surface properties was dependent on the culture media. There was no relationship between binding to immobilized collagen and binding to soluble 125I-labeled collagen. Particle-agglutination reactivity differed when using various collagen-coated microbead preparations. There were general differences in the particle-agglutination reactivity when collagen-coated latex beads were prepared using different coating procedures. The negative charge and hydrophobicity of the various collagen-coated microbead preparations were also studied by partitioning in the two-phase system of polyethylene glycol and dextran. Under these conditions, the alpha1+alpha2 collagen-chain mixture covalently immobilized on carboxy-modified latex beads was less hydrophobic and negatively charged than gelatin and native collagen immobilized on the same kind of latex beads. For latex beads passively coated with collagen preparations, the alpha1+alpha2 collagen-chain mixture was more hydrophobic than gelatin and native collagen. We suggest that for screening collagen-binding among Vibrio and Aeromonas strains, a reliable and sensitive particle-agglutination assay should consider the collagen preparation and the coating procedure for the immobilization of collagen onto the latex beads. In this regard, carboxy-modified latex beads coated with an alpha1+alpha2 collagen-chain mixture gave the best results.

Publiceringsår

1995

Språk

Engelska

Sidor

223-230

Publikation/Tidskrift/Serie

Archives of Microbiology

Volym

164

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

Springer

Ämne

  • Microbiology in the medical area

Nyckelord

  • Key words Cell-surface hydrophobicity . Cell-surface charge . Collagen . Aeromonas caviae . Aeromonas . hydrophila . Aeromonas sobria . Vibrio anguillarum . Vibrio cholerae

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0302-8933