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Presence of a novel form of phosphatidylinositol 4-kinase in rat liver

Författare:
  • Henric Olsson
  • Wilma Martinez
  • Bjørn K Drøbak
  • Bengt Jergil
Publiceringsår: 1995
Språk: Engelska
Sidor: 282-286
Publikation/Tidskrift/Serie: FEBS Letters
Volym: 361
Nummer: 2-3
Dokumenttyp: Artikel
Förlag: Elsevier

Sammanfattning

Rat liver microsomes contain two distinct forms of PtdIns 4-kinase which were resolved by heparin-Sepharose chromatography. One enzyme was identified as the type II PtdIns kinase previously isolated from exocytotic vesicles. The other enzyme, however, was a novel PtdIns 4-kinase isoform with properties differing from any other PtdIns kinase so far characterized. Both kinases were recognized by a monoclonal antibody specific for type II PtdIns 4-kinase, but the novel enzyme was considerably less sensitive to inhibition by adenosine and Ca2+ than type II enzymes, and in addition was specifically inhibited by submillimolar concentrations of dithioerythritol. The presence of a novel PtdIns 4-kinase isoform in rat liver raises the question of whether this enzyme is unique for this organ or whether it has a more widespread distribution but so far has avoided detection.

Disputation

Nyckelord

  • Chemistry
  • Signal transduction
  • Phosphoinositide
  • Phosphatidyl inositol 4-kinase
  • Rat liver

Övriga

Published
Yes
  • ISSN: 0014-5793

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