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The Yersinia HPI is present in Serratia liquefaciens isolated from meat.

In English

Författare

Summary, in English

Aims: The aim of the study was to screen the Enterobacteriaceae flora of meat for the presence of bacteria harbouring the Yersinia high-pathogenicity island (HPI).



Methods and Results: Bacteria from 29 meat and 29 liver samples were isolated on violetred bile glucose agar. A total of 197 isolates were screened for the presence of the irp2 gene, encoded within the HPI, by PCR. One isolate that was positive for irp2 gene was also positive for the fyuA, irp1, ybtP/ybtQ, ybtX/ybtS and int/asn tRNA genes by PCR. The presence of fyuA, irp1 and irp2 genes was confirmed by Southern hybridization.



Conclusions: The isolate was identified as Serratia liquefaciens by sequencing of the 16S rRNA gene and by ribotyping.



Significance and Impact of the Study: This is the first report of a Serratia harbouring the Yersinia HPI. Serratia is a frequently occurring Enterobacteriaceae genus in chill-stored meat.

Avdelning/ar

  • Department of Food Technology, Engineering and Nutrition

Publiceringsår

2003

Språk

Engelska

Sidor

275-280

Publikation/Tidskrift/Serie

Letters in Applied Microbiology

Volym

37

Issue

4

Dokumenttyp

Artikel i tidskrift

Förlag

Wiley-Blackwell

Ämne

  • Food Engineering

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0266-8254