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A metal binding site in the catalytic subunit of anaerobic ribonucleotide reductase.

In English

Författare

  • Derek Logan
  • E Mulliez
  • K-M Larsson.
  • S Bodevin
  • M Atta
  • P E Garnaud
  • B-M Sjöberg
  • M Fontecave

Summary, in English

A Zn(Cys)4 center has been found in the C-terminal region of the crystal structure of the anaerobic class III ribonucleotide reductase (RNR) from bacteriophage T4. The metal center is structurally related to the zinc ribbon motif and to rubredoxin and rubrerythrin. Mutant enzymes of the homologous RNR from Escherichia coli, in which the coordinating cysteines, conserved in almost all known class III RNR sequences, have been mutated into alanines, are shown to be inactive as the result of their inability to generate the catalytically essential glycyl radical. The possible roles of the metal center are discussed in relationship to the currently proposed reaction mechanism for generation of the glycyl radical in class III RNRs.

Publiceringsår

2003

Språk

Engelska

Sidor

3826-3831

Publikation/Tidskrift/Serie

Proceedings of the National Academy of Sciences

Volym

100

Issue

7

Dokumenttyp

Artikel i tidskrift

Förlag

National Academy of Sciences

Ämne

  • Biological Sciences

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1091-6490