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SufA - a bacterial enzyme that cleaves fibrinogen and blocks fibrin network formation.

In English

Författare

Summary, in English

Finegoldia magna is a member of the normal human bacterial flora on the skin and other non-sterile body surfaces, but this anaerobic coccus is also an important opportunistic pathogen. SufA was the first F. magna proteinase to be isolated and characterized. Many bacterial pathogens interfere with different steps of blood coagulation, and here we describe how purified SufA efficiently and specifically cleaves fibrinogen in human plasma. SufA is both secreted by F. magna and associated with the bacterial surface. Successful gene targeting has previously not been performed in anaerobic cocci, but in order to study the role of the SufA that is present at the bacterial surface, we constructed an F. magna mutant that expresses a truncated SufA lacking proteolytic activity. In contrast to wild-type bacteria that delayed the coagulation of human plasma, mutant bacteria had no such effect. Wild-type and mutant bacteria adhered to keratinocytes equally well, but in a plasma environment only wild-type bacteria blocked the formation of fibrin networks surrounding adherent bacteria. The effective cleavage of fibrinogen by SufA suggests that the interference with fibrin network formation represents an adaptive mechanism of F. magna with potential implications also for pathogenicity.

Publiceringsår

2009

Språk

Engelska

Sidor

238-248

Publikation/Tidskrift/Serie

Microbiology

Volym

155

Issue

Pt 1

Dokumenttyp

Artikel i tidskrift

Förlag

MAIK Nauka/Interperiodica

Ämne

  • Infectious Medicine
  • Dermatology and Venereal Diseases

Status

Published

Forskningsgrupp

  • Infection and immunomodulation

ISBN/ISSN/Övrigt

  • ISSN: 1465-2080