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Mass overloading in the flow field-flow fractionation channel studied by the behaviour of the ultra-large wheat protein glutein.

In English

Författare

Summary, in English

Flow field-flow fractionation (FFF) has previously been used in successful fractionation and characterisation of the ultra-large wheat protein glutenin. The many parameters, which may influence the retention behaviour, especially when analysing extremely high-molecular-mass samples such as glutenin, are here reported. Size determination from the sample retention time, using FFF theory, will as a result have a very low accuracy. The need for direct molecular mass determination, such as by light scattering, in combination with FFF, in order to do accurate size measurements of glutenin is pointed out as well as the importance to minimise the overloading.

Publiceringsår

2003

Språk

Engelska

Sidor

99-109

Publikation/Tidskrift/Serie

Journal of Chromatography A

Volym

1011

Issue

1-2

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Analytical Chemistry

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0021-9673