Meny

Javascript verkar inte påslaget? - Vissa delar av Lunds universitets webbplats fungerar inte optimalt utan javascript, kontrollera din webbläsares inställningar.
Du är här

Buffer medium exchange in continuous cell and particle streams using ultrasonic standing wave focusing

Författare:
Publiceringsår: 2009
Språk: Engelska
Sidor: 269-277
Publikation/Tidskrift/Serie: Microchimica Acta
Volym: 164
Nummer: 3-4
Dokumenttyp: Artikel
Förlag: Springer Wien

Sammanfattning

A microfluidic strategy to perform buffer exchange of particle and cell suspensions in a continuous flow format on, chip is presented. Ultrasonic standing wave technology is utilized to confine particulate matter to the centre of a buffer exchange channel while particle free buffer is sequentially aspirated via capillaries that branch off from the buffer exchange channel. At each such branch, clean buffer is supplied at an equal flow-rate from a capillary at the opposing channel wall, generating a sideways translation of the original buffer, laminated with a wash buffer stream. Each such junction increases the buffer exchange ratio accordingly. The reported buffer exchange system provides means to adjust buffer exchange conditions on-line by tuning the ratio of the cross-flow wash buffer relative the sample suspension flow, rate. The system performance was evaluated using 5 mu m polystyrene microbeads and a dye as the model contaminant. Wash efficiencies up to 96.4% were accomplished with a 0.2% solid content bead suspension, using eight cross-flow junctions, effectively exchanging the carrier buffer twice. The corresponding data for erythrocyte washing was recorded to be 98.3% at a haematocrit of 2%.

Disputation

Nyckelord

  • Technology and Engineering
  • Buffer medium exchange
  • radiation force
  • Acoustic
  • Erythrocytes
  • Ultrasonic standing wave
  • Particle washing

Övriga

Published
Yes
  • ISSN: 0026-3672

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen