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Integrated protein microchip assay with dual fluorescent- and MALDI read-out

Publiceringsår: 2004
Språk: Engelska
Sidor: 988-994
Publikation/Tidskrift/Serie: Journal of Proteome Research
Volym: 3
Nummer: 5
Dokumenttyp: Artikel
Förlag: American Chemical Society


A pore chip protein array (PCPA) concept based on a dual readout configuration, fluorescence imaging, and MALDI-TOF MS has been developed. Highly packed, (>4000 Spots/cm(2)), antibody arrays were dispensed on the porous chip by using a piezo-electric microdispenser. Sandwich assay was made after blocking by addition of a secondary antibody either lgG-FITC-labeled or anti-Ang II. The antigen in the first system was a large protein (lgG), and in the other system, a FITC marked peptide Angiotensin II (Ang II) was used. Ang II antibodies showed specificity for Ang II, while the Ang I antibodies showed binding properties for Ang I, II, and Renin. Fluorescence and MALDI TOF MS read-out was made for lgG and Ang II. A major advantage of the dual read-out PCPA approach is that both affinity binding and mass identity are derived. Detection limits for Ang II on the chip is as low as 500 zmol (Ang II).



  • Analytical Chemistry
  • Medical Engineering
  • matrix-assisted laser desorption ionization time-of-flight


  • ISSN: 1535-3893

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