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Glutamate oxidase advances the selective bioanalytical detection of the neurotoxic amino acid beta-ODAP in grass pea: A decade of progress

In English

Författare

Summary, in English

The search for an enzyme as a reagent for selective bioanalytical detection of the neurotoxic amino acid, beta-N-oxalyl-L-,alpha,beta-diaminopropionic acid, beta-ODAP (found in grass pea, Lathyrus sativus) led to its redox catalytic reaction by glutamate oxidase (GluOx). Homogeneous kinetic studies and an immobilized GluOx reactor-based flow-injection assay were initially made for beta-ODAP with small immobilized GluOx/catalase glutamate-destroying prereactors. The method was applied to examine the toxin content in processed grass pea. The kinetics and the equilibrium of the thermal isomerization of beta-ODAP to the nontoxic isomer alpha-ODAP established that GluOx is specific to the neurotoxin. The first ever GluOx-based amperometric biosensor for liquid chromatography (LC) detection was reported in 1997. This biosensor coupled with a refractive index detector improved LC performance. The most recent work with GluOx resulted in MnO2-based screen-printed amperometric biosensor, with offline elimination of glutamate interference by glutamate decarboxylase. A single-shot chemiluminescent sensor developed for hydrogen peroxide is also proposed for beta-ODAP with GluOx application. This decade of progress resulted from studies that included four Ph.D. (Ethiopia, Sweden, Austria), four M.Sc. (Ethiopia, Sweden) and Licentiate (Sweden) theses projects, plus one collaborative project in Sweden. The advances in grass pea research may be regarded as a model north-south cooperation for research and education.

Publiceringsår

2004

Språk

Engelska

Sidor

765-775

Publikation/Tidskrift/Serie

Pure and Applied Chemistry

Volym

76

Issue

4

Dokumenttyp

Artikel i tidskrift

Förlag

IUPAC

Ämne

  • Analytical Chemistry
  • Nutrition and Dietetics

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0033-4545