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Importance of polyamines in cell cycle kinetics as studied in a transgenic system.

Författare:
Publiceringsår: 2005
Språk: Engelska
Sidor: 254-264
Publikation/Tidskrift/Serie: Exp Cell Res
Volym: 308
Nummer: May 26
Dokumenttyp: Artikel
Förlag: Academic Press

Sammanfattning

Polyamines are organic cations, which are considered essential for normal cell cycle progression. This view is based on results from numerous studies using a variety of enzyme inhibitors or polyamine analogues interfering with either the metabolism or the physiological functions of the polyamines. However, the presence of non-specific effects may be hard to rule out in such studies. In the present study, we have for the first time used a transgenic cell system to analyze the importance of polyamines in cell growth. We have earlier shown that expression of trypanosomal ODC in an ODC-deficient variant of CHO cells (C55.7) supported growth of these otherwise polyamine auxotrophic cells. However, one of the transgenic cell lines grew much slower than the others. As shown in the present study, the level of ODC activity was much lower in these cells, and that was reflected in a reduction of cellular polyamine levels. Analysis of cell cycle kinetics revealed that reduction of growth was correlated to prolongation of the G(1), S, and G(2) + M phases in the cells. Providing exogenous putrescine to the cells resulted in a normalization of polyamine levels as well as cell cycle kinetics indicating a causal relationship.

Disputation

Nyckelord

  • Medicine and Health Sciences
  • Biology and Life Sciences
  • polyamines
  • ornithine decarboxylase
  • trypanosomal
  • cyclins
  • cell growth
  • cell cycle

Övriga

Published
Yes
  • ISSN: 0014-4827

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