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Microarrays based on affinity-tagged SCFV antibodies: Sensitive detection of analyte in complex proteomes

Författare:
Publiceringsår: 2005
Språk: Engelska
Sidor: 264-266
Publikation/Tidskrift/Serie: Micro Total Analysis Systems 2004, Vol 2
Nummer: 297
Dokumenttyp: Konferensbidrag
Förlag: Royal Society of Chemistry

Sammanfattning

Antibody microarray is a novel technology that will allow us to efficiently perform global proteome analysis. Performing rapid and multiplexed analysis on complex proteomes, such as human serum, targeting also low-abundant analytes will, however, place high demands upon the microarray design with respect to logistics, specificity and sensitivity. In this study, we have shown that non-purified affinity-tagged scFv antibody fragments could be directly applied as probes thereby eliminating the need for any time-consuming pre-purification steps. Further, highly functional arrays were obtained providing an assay sensitivity in the pM to fM range. In fact, 300 zeptomole analyte (approx. 5,000 molecules) may be sufficient for detection. Moreover, highly complex proteomes could be analysed without impairing the specificity and sensitivity of the setups. This is a pre-requisite for the design of high-density antibody arrays applied in high-throughput proteomics.

Disputation

Nyckelord

  • Chemistry
  • Medicine and Health Sciences
  • scFv
  • antibody microarrays
  • antibody chips
  • sensitivity
  • affinity-tag

Övriga

8th International Conference on Miniaturized Systems for Chemistry and Life Sciences
2014-09-28
Malmo, Sweden
Published
Yes
  • ISSN: 0260-6291
  • ISBN: 9780854048960

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