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Profiling of internalizing tumor-associated antigens on breast and pancreatic cancer cells by reversed genomics

Författare:
Publiceringsår: 2004
Språk: Engelska
Sidor: 235-242
Publikation/Tidskrift/Serie: Cancer Letters
Volym: 208
Nummer: 2
Dokumenttyp: Artikel
Förlag: Elsevier Ltd

Sammanfattning

Human antibodies directed towards functionally associated tumor antigens have great potentials as adjuvant treatment in cancer therapy. Here we describe an efficient subtractive approach to select single chain Fv (scFv) antibodies, specifically binding to unknown rapidly internalizing tumor-associated antigens (TAA) on human breast and pancreatic carcinoma cell lines. After re-engineering the scFv into intact IgG molecules, these fully human antibodies displayed individual binding profiles to TAAs on breast, pancreatic, colorectal and prostate carcinomas, while showing no reactivity to lymphomas. The ability of the selected antibodies to undergo receptor-mediated internalization was verified by confocal microscopy, thus proving our strategy to provide a unique set of human antibodies, potentially useful in immunotherapy. (C) 2003 Elsevier Ltd. All rights reserved.

Disputation

Nyckelord

  • Chemistry

Övriga

Published
Yes
  • ISSN: 0304-3835

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