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Size and Structure Characterization of Ethylhydroxyethyl Cellulose by the Combination of Field-Flow Fractionation with Other Techniques. Investigation of Ultralarge Components

In English

Författare

Summary, in English

Ethylhydroxyethyl cellulose (EHEC) of three different viscosity classes (EHEC I, II, and III) was analyzed by programmed cross-flow asymmetrical flow field-flow fractionation coupled to multiangle light scattering and refractive index detectors to determine their size and molar mass distribution. Two size populations were detected in the two lower viscosity classes, EHEC I and II, one high molar mass and one ultrahigh molar mass (UHM). The two covered molar masses from 104 up to 109 g·mol-1. The highest viscosity class EHEC III was less size-dispersed covering molar masses from 5 × 105 to 5 × 107 g·mol-1. Filtering of the EHEC II solution removed small amounts of compact UHM material. Enzyme treatments were performed on EHEC II to further characterize it. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and anion ion-exchange chromatography coupled to pulsed amperometric detection showed that the UHM component contained EHEC.

Publiceringsår

2004

Språk

Engelska

Sidor

97-105

Publikation/Tidskrift/Serie

Biomacromolecules

Volym

5

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

The American Chemical Society (ACS)

Ämne

  • Analytical Chemistry
  • Biological Sciences

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1526-4602