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Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

Författare:
Publiceringsår: 2009
Språk: Engelska
Sidor: 469-479
Publikation/Tidskrift/Serie: Planta
Volym: 230
Nummer: 3
Dokumenttyp: Artikel
Förlag: Springer

Sammanfattning

Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.

Disputation

Nyckelord

  • Biology and Life Sciences
  • Electroporation
  • Cell membrane recovery
  • Metabolic analysis
  • multivariate data analysis
  • OPLS
  • Wounding
  • Stress response

Övriga

Published
Yes
  • ISSN: 0032-0935

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