Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy
Författare
Summary, in English
A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.
Avdelning/ar
Publiceringsår
2002
Språk
Engelska
Sidor
349-354
Publikation/Tidskrift/Serie
Mycological Research
Volym
106
Issue
3
Fulltext
- Available as PDF - 334 kB
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Dokumenttyp
Artikel i tidskrift
Förlag
Cambridge University Press
Ämne
- Biological Sciences
Status
Published
Forskningsgrupp
- Microbial Ecology
ISBN/ISSN/Övrigt
- ISSN: 0953-7562