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Absence of adenosine A(1) receptors unmasks pulses of insulin release and prolongs those of glucagon and somatostatin.

In English

Författare

  • S Albert Salehi
  • Fariborz Parandeh
  • Bertil B Fredholm
  • Eva Grapengiesser
  • Bo Hellman

Summary, in English

AIMS: Extracellular ATP modulates pulsatile release of insulin, glucagon and somatostatin by activating P2Y(1) receptors. The present study examines if adenosine via A(1) receptors (A(1)R) interferes with pulsatile islet hormone release. MAIN METHODS: Pancreas was perfused in mice expressing or lacking the A(1) receptor and the hormones measured with radioimmunoassay. Cytoplasmic Ca(2+) was recorded in isolated beta-cells using the fura-2 indicator. KEY FINDINGS: Addition of 10microM adenosine removed the Ca(2+) transients supposed to coordinate the insulin release pulses. This effect of adenosine was counteracted by 100nM of the A(1)R antagonist DPCPX. In situ perfusion of the pancreas indicated two phases of islet hormone release when glucose was raised from 3.3 to 16.7mM. The first phase was characterized by a brief dip followed by a peak, which was more pronounced for insulin and somatostatin than for glucagon. The second phase was markedly affected by knock out of A(1)R. The wild-type A(1)R (+/+) mice, usually lacked statistically verified insulin pulses but generated antisynchronous glucagon and somatostatin pulses with half-widths of 4min. In the A(1)R (-/-) mice time-average release of insulin during the second phase was almost three times higher than in the controls and 30% of the hormone was released as distinct pulses with half-widths of 3min. The absence of the A(1)R receptor resulted in 50% prolongation of the pulse cycles of glucagon and somatostatin and loss of their antisynchronous relationship. SIGNIFICANCE: The A(1)R receptor is important both for the amplitude (insulin) and duration (glucagon and somatostatin) of islet hormone pulses.

Avdelning/ar

  • Islet cell physiology

Publiceringsår

2009

Språk

Engelska

Sidor

470-476

Publikation/Tidskrift/Serie

Life Sciences

Volym

85

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Endocrinology and Diabetes

Status

Published

Forskningsgrupp

  • Islet cell physiology

ISBN/ISSN/Övrigt

  • ISSN: 1879-0631