Complex Nature of the Genome in a Wine-spoilage Yeast, Dekkera bruxellensis.

Summary, in English

When the genome organization of thirty native isolates belonging to a wine-spoilage yeast Dekkera/Brettanomyces bruxellensis, a distant relative of Saccharomyces cerevisiae, was examined, the number of chromosomes varied drastically, from 4 to at least 9. When single gene probes were used in Southern analysis, the corresponding genes usually mapped to at least two chromosomal bands, excluding a simple haploid organization of the genome. When different loci were sequenced, in most cases several different haplotypes were obtained in each single isolate, and they belonged to two sub-types. Phylogenetic reconstruction using haplotypes revealed that the sequences from different isolates, belonging to one sub-type, were more similar to each other than to the sequences belonging to the other sub-type, within the isolate. Reanalysis of the genome sequence confirmed that also the partially sequenced strain Y879 is not a simple hybrid and its genome contains of app. 1% polymorphic sites. The present situation could be explained by (i) a hybridization event where two similar but different genomes have recently fused together or, (ii) that the diploid progenitor of all analyzed strains lost a regular sexual cycle and the genome started to accumulate mutations.

• Författare: Linda Hellborg; Jure Piskur
• Avdelning/ar: Biologiska institutionen; Molekylär cellbiologi
• Publiceringsår: 2009
• Språk: Engelska
• Sidor: 1739-1749
• Publikation/Tidskrift/Serie: Eukaryotic Cell
• Volym: 8
• Issue: 11
• Länkar: http://dx.doi.org/10.1128/EC.00115-09
• Dokumenttyp: Artikel i tidskrift
• Förlag: American Society for Microbiology
• Ämne: Biological Sciences
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1535-9778