Acetylation of intragenic histones on HPV16 correlates with enhanced HPV16 gene expression.
Författare
Summary, in English
We report that many histone modifications are unevenly distributed over the HPV16 genome in cervical cancer cells as well as in HPV16-immortalized keratinocytes. For example, H3K36me3 and H3K9Ac that are common in highly expressed cellular genes and over exons, were more common in the early than in the late region of the HPV16 genome. In contrast, H3K9me3, H4K20me3, H2BK5me1 and H4K16Ac were more frequent in the HPV16 late region. Furthermore, a region encompassing the HPV16 early polyadenylation signal pAE displayed high levels of histone H3 acetylation. Histone deacetylase (HDAC) inhibitors caused a 2- to 8-fold induction of HPV16 early and late mRNAs in cervical cancer cells and in immortalized keratinocytes, while at the same time increasing the levels of acetylated histones in the cells and on the HPV16 genome specifically. We concluded that increased histone acetylation on the HPV16 genome correlates with increased HPV16 gene expression.
Avdelning/ar
Publiceringsår
2015
Språk
Engelska
Sidor
244-259
Publikation/Tidskrift/Serie
Virology
Volym
482
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Microbiology in the medical area
Status
Published
Forskningsgrupp
- Experimental Infection Medicine, Malmö
- Molecular virology
ISBN/ISSN/Övrigt
- ISSN: 1096-0341