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Proton NMR of (15)N-Choline Metabolites Enhanced by Dynamic Nuclear Polarization.

In English

Författare

  • Riddhiman Sarkar
  • Arnaud Comment
  • Paul R Vasos
  • Sami Jannin
  • Rolf Gruetter
  • Geoffrey Bodenhausen
  • Hélène Hall
  • Deniz Kirik
  • Vladimir Denisov

Summary, in English

Chemical shifts of protons can report on metabolic transformations such as the conversion of choline to phosphocholine. To follow such processes in vivo, magnetization can be enhanced by dynamic nuclear polarization (DNP). We have hyperpolarized in this manner nitrogen-15 spins in (15)N-labeled choline up to 3.3% by irradiating the 94 GHz electron spin resonance of admixed TEMPO nitroxide radicals in a magnetic field of 3.35 T during ca. 3 h at 1.2 K. The sample was subsequently transferred to a high-resolution magnet, and the enhanced polarization was converted from (15)N to methyl- and methylene protons, using the small (2,3)J((1)H,(15)N) couplings in choline. The room-temperature lifetime of nitrogen polarization in choline, T(1)((15)N) approximately 200 s, could be considerably increased by partial deuteration of the molecule. This procedure enables studies of choline metabolites in vitro and in vivo using DNP-enhanced proton NMR.

Publiceringsår

2009

Språk

Engelska

Sidor

16014-16014

Publikation/Tidskrift/Serie

Journal of the American Chemical Society

Volym

131

Issue

44

Dokumenttyp

Artikel i tidskrift

Förlag

The American Chemical Society (ACS)

Ämne

  • Neurosciences

Status

Published

Forskningsgrupp

  • Brain Repair and Imaging in Neural Systems (BRAINS)

ISBN/ISSN/Övrigt

  • ISSN: 1520-5126