Meny

Javascript is not activated in your browser. This website needs javascript activated to work properly.
Du är här

Single cell analysis of the common lymphoid progenitor compartment reveals functional and molecular heterogeneity.

Författare:
Publiceringsår: 2010
Språk: Engelska
Sidor: 2601-2609
Publikation/Tidskrift/Serie: Blood
Volym: 115
Nummer: 13
Dokumenttyp: Artikel
Förlag: American Society of Hematology

Sammanfattning

In order to investigate molecular events involved in the regulation of lymphoid lineage commitment, we crossed lambda5 reporter transgenic mice to Rag1-GFP knock in mice. This allowed us to sub-fractionate common lymphoid progenitors (CLPs) and pre-pro-B (Fraction A) cells into lambda5(-)Rag1(low), lambda5(-)Rag1(high) and lambda5(+)Rag1(high) cells. Clonal in vitro differentiation analysis demonstrated that Rag1(low) cells gave rise to B/T and NK cells. Rag1(high) cells displayed reduced NK-cell potential with preserved capacity to generate B- and T-lineage cells, while the lambda5(+) cells were B-lineage restricted. Ebf1 and Pax5 expression was largely confined to the Rag1(high) populations. These cells also expressed a higher level of the surface protein LY6D, providing an additional tool for the analysis of early lymphoid development. These data suggest that the classical CLP compartment comprises a mixture of cells with relatively restricted lineage potentials, thus opening new possibilities to investigate early hematopoiesis.

Disputation

Nyckelord

  • Medicine and Health Sciences

Övriga

Published
Yes
  • ISSN: 1528-0020

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen