Efficient transduction of neurons using Ross River glycoprotein-pseudotyped lentiviral vectors.
Författare
Summary, in English
Lentiviral vectors are promising tools for CNS gene transfer since they efficiently transduce the cells of the nervous system in vivo. In this study, we have investigated the transduction efficiency of lentiviral vectors pseudotyped with Ross River virus glycoprotein (RRV-G) (RRV-G-pseudotyped lentiviral vectors (RRV-LV)). The RRV is an alphavirus with an extremely broad host range, including the cells of the central nervous system. Previous studies have shown that lentiviral vectors can be efficiently pseudotyped with this envelope protein and have demonstrated promising features of such vectors, including the possibility to establish stable producer cell lines. After injection of RRV-LV expressing green fluorescent protein into different structures in the rat brain we found efficient transduction of both neurons and glial cells. By using two cell-type-specific promoters, neuron-specific enolase and human glial fibrillary acidic protein, we demonstrated cell-specific transgene expression in the desired cell type. Ross River virus glycoprotein-pseudotyped lentiviral vectors also transduced human neural progenitor cells in vitro, showing that receptors for the RRV-G are present on human neural cells.
Avdelning/ar
Publiceringsår
2006
Språk
Engelska
Sidor
966-973
Publikation/Tidskrift/Serie
Gene Therapy
Volym
13
Issue
12
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Nature Publishing Group
Ämne
- Neurosciences
Nyckelord
- green fluorescent protein
- alphavirus
- astrocyte
- gene transfer
Status
Published
Forskningsgrupp
- Neurobiology
- CNS Gene Therapy
ISBN/ISSN/Övrigt
- ISSN: 0969-7128